TrialtusBioscience23 [Trialtus Bioscience] Im7 4B Gravity Flow Column [Trialtus Bioscience] Im7 4B Gravity Flow Column 11-2060(1mL) 11-2065(5mL) Gravity flow columns are packed with our Im7 4B Resin for use with proteins tagged with CL7. Columns are available in 1 ml and 5 ml sizes. Resin upholds its effectiveness for multiple reactivations. TriAltus’ Im7 4B Resin retains a 1:1 molar binding ratio during repeated uses. The Im7 4B Resin has been used to purify a va.. 2023. 5. 4. [Trialtus Bioscience] SUMO Protease [Trialtus Bioscience] SUMO Protease SKU | 1 mg: 30-1030 5 mg: 30-1035 10 mg: 30-1040 50 mg: 30-1046 100 mg: 30-1050 The SUMO protease releases target proteins from TriAltus’s Im7 resin. This protease recognizes SUMO tertiary structure – not a specific amino acid sequence – to cleave and release the target protein from the Im7-bound CL7 tag. PURITY | ~100% (SDS gel analysis) ENZYME SOURCE | Yeast.. 2023. 4. 20. [Trialtus Bioscience] Im7 4B Resin [Trialtus Bioscience] Im7 4B Resin SKU | 1 mL: 11-1060 2 mL: 11-1062 5 mL: 11-1065 15mL: 11-1071 The Im7 Resin contains a highly specific, resilient Im7 domain that binds the CL7 tag (~16 kDa) fused to target proteins. Following cleavage with the appropriate protease, the target protein releases from the Im7-bound CL7. Available in 1-, 2-, 5-, and 15-mL volumes of settled resin (50% suspension)... 2023. 4. 10. [Trialtus Bioscience] Plasmid #34 [Trialtus Bioscience] Plasmid #34 SKU: 20-1034 EXPRESSION Transcription is induced with IPTG and driven by the T7 RNA polymerase. The plasmid is designed for expression in E. coli. PEPTIDE TAG The C-terminal CL7 tag is downstream of Sortase A (SRT) and PreScission protease (PSC) cleavage sites and a His8 tag. CLEAVAGE SITE(S) N-terminal SUMO and C-terminal SRT and PSC cleavage sites are availabl.. 2023. 4. 4. [Trialtus Bioscience] Starter Kit: 1-mL Im7 4B Gravity Flow Column [Trialtus Bioscience] Starter Kit: 1-mL Im7 4B Gravity Flow Column The 1-mL Im7 4B Resin Gravity Flow Column Starter Kit provides all essential buffers and reagents necessary for the purification of CL7 tagged proteins. Please select a plasmid and an elution protease. -- The Im7 4B 1-mL Gravity Flow Column Starter Kit consists of: Equilibration, Lysis, Elution Buffer (100 mL) Wash Buffer A1 (Hig.. 2023. 3. 27. [Trialtus Bioscience] Plasmid #10 [Trialtus Bioscience] Plasmid #10 SKU: 20-1010 Plasmid #10 allows for the purification of membrane proteins. It includes an N-terminal His8 tag, a C-terminal CL7 tag, and an engineered signal peptide from the Bacillus halodurans YidC gene. Membrane proteins are inserted at the BamHI/SpeI restriction sites, where there is an existing 1711 base pair restriction fragment. EXPRESSION Transcription i.. 2023. 2. 22. [Trialtus Bioscience] Starter Kit: 1-mL Im7 4B Gravity Flow Column [Trialtus Bioscience] Starter Kit: 1-mL Im7 4B Gravity Flow Column The 1-mL Im7 4B Resin Gravity Flow Column Starter Kit provides all essential buffers and reagents necessary for the purification of CL7 tagged proteins. Please select a plasmid and an elution protease. -- The Im7 4B 1-mL Gravity Flow Column Starter Kit consists of: Equilibration, Lysis, Elution Buffer (100 mL) Wash Buffer A1 (Hig.. 2023. 1. 31. 이전 1 2 3 다음
