[Trialtus Bioscience] Plasmid #34

[Trialtus Bioscience] Plasmid #34

 

SKU: 20-1034

 

EXPRESSION
Transcription is induced with IPTG and driven by the T7 RNA polymerase. The plasmid is designed for expression in E. coli.

PEPTIDE TAG
The C-terminal CL7 tag is downstream of Sortase A (SRT) and PreScission protease (PSC) cleavage sites and a His8 tag. 

CLEAVAGE SITE(S)
N-terminal SUMO and C-terminal SRT and PSC cleavage sites are available in Plasmid #34. 

OTHER TAGS
The encoded region begins with a Trx tag and includes a C-terminal His8 tag upstream of CL7. 

 

CLONING OPTIONS
1. HindIII/SpeI Insertion Site – Trx | SUMO | Gene of Interest | SRT | PSC | PSC | PSC | His8 | CL7

This insertion option results in 7 extra aa at the N terminus of the target following SUMO protease cleavage. 

2. KpnI/SpeI Insertion Site – Trx | SUMO | Gene of Interest | SRT | PSC | PSC | PSC | His8 | CL7

This insertion option results in 2 extra aa at the N terminus of the target following SUMO protease cleavage. 

3. Bsu36I/SpeI Insertion Site – Trx | SUMO | Gene of Interest | SRT | PSC | PSC | PSC | His8 | CL7

This insertion option maintains the Gene of Interest’s wildtype sequence, without adding any extra residues. The N-terminus of the Gene of Interest must include the following to complete the SUMO C-terminal sequence:

EXPIRATION | 6 months from receipt when stored as directed

Plasmid Sequences are available on the Plasmid Guide Page

Use of TriAltus products falls under our limited license agreement and is for non-profit research use only. For commercial use, please contact us for licensing.

 

Plasmid #34 enables target proteins to be labeled with C-terminal His8 and CL7. Protease cleavage removes both tags.