TrialtusBioscience22 [Trialtus Bioscience] CRISPR-Cas9 Protein [Trialtus Bioscience] CRISPR-Cas9 ProteinSKU |100 µg: 40-1320500 µg: 40-1325 TriAltus' highly pure Cas9 protein is used for genome editing with CRISPR technology. Recombinant Streptococcus pyogenes Cas9 (wt) protein is expressed in E. coli with two (N-terminal and C-terminal) nuclear localization signals (NLS) and the CL7 tag, which is removed with PreScission protease during the purification p.. 2024. 11. 7. [Trialtus Bioscience] SUMO Protease [Trialtus Bioscience] SUMO Protease SKU | 1 mg: 30-11305 mg: 30-113510 mg: 30-114050 mg: 30-1146100 mg: 30-1150 The SUMO protease releases target proteins from TriAltus’s Im7 resin. This protease recognizes SUMO tertiary structure – not a specific amino acid sequence – to cleave and release the target protein from the Im7-bound CL7 tag. PURITY | ~100% (SDS gel analysis)ENZYME SOURCE | Yeast pr.. 2024. 10. 7. [Trialtus Bioscience] Im7 6B FPLC Column [Trialtus Bioscience] Im7 6B FPLC Column SKU |1 mL: 10-30605 mL: 10-3065 FPLC columns are packed with our Im7 6B Resin for use with proteins tagged with CL7. Columns are available in 1 ml and 5 ml sizes.Resin upholds its effectiveness for multiple reactivations. TriAltus’ Im7 6B Resin retains a 1:1 molar binding ratio during repeated uses. The Im7 6B Resin has been used to purify a variety of p.. 2024. 7. 15. [Trialtus Bioscience] Gentle Elution Buffer [Trialtus Bioscience] Gentle Elution Buffer SKU |250 mL: 50-27831 L: 50-2790Gentle Elution Buffer allows for elution of CL7-tagged proteins from Im7 resin and/or regeneration of Im7 resin without denaturing effects. The high salt and near-neutral pH of Gentle Elution Buffer maintains the native structure of the target protein and the Im7 resin ligand without need for subsequent refolding steps... 2024. 7. 2. [Trialtus Bioscience] Starter Kit: 1-mL Im7 4B Gravity Flow Column [Trialtus Bioscience] Starter Kit: 1-mL Im7 4B Gravity Flow ColumnThe 1-mL Im7 4B Resin Gravity Flow Column Starter Kit provides all essential buffers and reagents necessary for the purification of CL7 tagged proteins. Please select a plasmid and an elution protease.--The Im7 4B 1-mL Gravity Flow Column Starter Kit consists of:Equilibration, Lysis, Elution Buffer (100 mL) Wash Buffer A1 (High S.. 2024. 5. 3. [Trialtus Bioscience] Plasmid #01 [Trialtus Bioscience] Plasmid #01 SKU: 20-1001 EXPRESSION Transcription is induced with IPTG and driven by the T7 RNA polymerase. The plasmid is designed for expression in E. coli. PEPTIDE TAG The CL7 tag for purification is fused to the C-terminus of the third subunit of the ttRNAP protein. CLEAVAGE SITE(S) Plasmid #1 includes several PreScission protease (PSC) cleavage sites. One is at the N-t.. 2024. 2. 2. [Trialtus Bioscience] Im7 4B FPLC Column [Trialtus Bioscience] Im7 4B FPLC Column SKU | 1 mL: 11-3060 5 mL: 11-10365 FPLC columns are packed with our Im7 4B Resin for use with proteins tagged with CL7. Columns are available in 1 ml and 5 ml sizes. Resin upholds its effectiveness for multiple reactivations. TriAltus’ Im7 4B Resin retains a 1:1 molar binding ratio during repeated uses. The Im7 4B Resin has been used to purify a variety o.. 2023. 11. 3. [Trialtus Bioscience] Im7 6B Resin [Trialtus Bioscience] Im7 6B Resin SKU | 1 mL: 10-1060 2 mL: 10-1062 5 mL: 10-1065 15 mL: 10-1071 BUFFER | 20 mM Tris-Cl pH 8.0, 0.5M NaCl, 5% glycerol, 0.05% sodium azide PARTICLE SIZE | Crosslinked agarose 6B beads (45 – 165 μM) PH STABILITY | The Im7 protein is stable on the beads at pH 3-10. However, CL7/Im7 binding is stable at pH 4.2 – 10 only. SALT STABILITY | ≤ 4M NaCl tested BINDING CAP.. 2023. 10. 13. 이전 1 2 3 다음
