[Hawach Scientific] C18-Aqueous HPLC Columns

[Hawach Scientific]  C18-Aqueous HPLC Columns

Column: XChroma HPLC Column, C18-Aqueous, 5μm, 4.0×150mm
Mobile Phase: Methanol/acetonitrile/water=38/38/24
Wavelength: 245nm
Temperature: 30°C
Flow Rate: 1mL/min
Injection Volume: 5μL

 

SLHC18A32130	HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x30mm	1
SLHC18A32133	HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x33mm	1
SLHC18A32150	HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x50mm	1
SLHC18A32175	HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x75mm	1
SLHC18A321100	HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x100mm	1
SLHC18A321125	HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x125mm	1
SLHC18A321150	HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x150mm	1
SLHC18A321200	HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x200mm	1
SLHC18A321250	HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x250mm	1
SLHC18A33030	HPLC Column, C18-Aqueous, 3μm, 120Å, 3.0x30mm	1
SLHC18A33050	HPLC Column, C18-Aqueous, 3μm, 120Å, 3.0x50mm	1
SLHC18A33075	HPLC Column, C18-Aqueous, 3μm, 120Å, 3.0x75mm	1

Descriptions of C18-Aqueous Liquid Chromatography Column

C18-Aqueous HPLC Column can endure 100% aqueous phase samples. With appropriate surface coverage and double end-capped technique, the C18-Aqueous liquid chromatography column has good compatibility with highly aqueous-based mobile phase and can provide a longer column lifetime. The stationary phase was specially made under the double end-capped technique making it stable and specially used for the aqueous-based mobile phase.

Advantage of C18 Aqueous HPLC columns

• The most widely applied C18-Aqueous HPLC column in the analysis of the food industry;
• 300 Å pore size available for the separation of macromolecular compounds;
• Highly specific surface area and mechanical strength ensured from high purity silica gel;
• Double end-capped for excellent peak shape when separation of polar, acidic, and basic compounds;
• Column peak shape and volume are perfect by spherical silica gel and good stability of batch to batch;
• Buffer systems and ion pair reagents do not need for the analysis of drugs, combined chemicals, and LC-MS applications.

Features

• Increase column retention for polar and water-soluble compounds;
• The fully end-capped stationary phase offers a longer column lifetime;
• Less retentive than C18 Universal for non-polar compounds;
• No phase collapse and especially suitable for high aqueous-based mobile phase;
• Excellent column peak shapes for basic and polar compounds are ensured by the fully end-capped stationary phase.

Bonded Phase	USP No.	Particle Size	Pore Size	Surface Area(m2)	Carbon Loading	pH range	End-capped
C18-aqueous	L1	3 μm, 5 μm, 10 μm	120Å	320	12%	1.5-10	Yes

Application

• Separation of metabolites and bio-molecules;
• Excellent column efficiency especially for applications of water-soluble compounds which cannot be retained on the traditional C18 stationary phase;
• Drug degradation products Separation such as water-soluble vitamins, small molecules, amino acids, and small molecules of the nucleoside.