[Tulip Diagnostics] ELISA for the detection of antibodies to HCV
QUALISA HCV kit contains,
• Microwell strips (3 x 8 wells/pouch)
• Reagents
• Accessories
96 Tests 40603096
192 Tests 40603192
480 Tests 40603480
Application
For detection of antibodies to HCV in human serum/plasma
Principle
Enzyme Linked Immunosorbent Assay (ELISA)
Sensitivity
100%
Specificity
100%
Reagents
3 x 8 Breakable microwells strips, coated with synthetic peptides representing core, NS3, NS4& NS5 regions of the HCV genome derived from multiple genotypes of HCV. Ready to use.
Conjugate: Agglutinating sera for Human IgGHRPO (Horse Radish Peroxidase) (50X). To be diluted 50 times with conjugate diluent. Conjugate diluent:
Buffered solution containing stabilizing proteins and preservatives. Ready to use. Sample diluent: Buffered solution containing stabilizing proteins and preservatives. Ready to use.
Substrate: Solution containing Tetramethyl benzidine(TMB) and hydrogen peroxide. Ready to use.
Wash buffer: Buffer contains surfactants (20 X). To be diluted 20 times with distilled or deionized water.
Stop solution: Diluted acid. Ready to use. Positive Control: Inactivated and stabilized human serum reactive for HCV and non-reactive for HIV & HBsAg with preservatives.
Negative control: Inactivated and stabilized human serum non-reactive for HIV-1 and HIV-2, HCV & HBsAg. Test duration 90 minutes
Storage / Stability 2-8°C, 12 months
