[CTK Biotech] Ferritin ELISA CE

[CTK Biotech] Ferritin ELISA CE

 

• Streptavidin Coated Microwells
• FTN Calibrators:
  • C1 (0 ng/mL)
  • C2 (10 ng/mL)
  • C3 (50 ng/mL)
  • C4 (150 ng/mL)
  • C5 (400 ng/mL)
  • C6 (800 ng/mL)
• FTN Assay Control
• FTN Enzyme Reagent
• FTN Biotin Reagent
• Wash Buffer (40X Concentrate)
• TMB Substrate
• Stop Solution
• Product Insert
• ELISA Working Sheet

• Sensitivity: Detects ferritin levels as low as 0.06 ng/mL at 2SD
• Specificity: No cross reactivity detected with ASA at 10 mg/mL, bilirubin at 10 μg/mL, FSH at 10,000 mIU/ML, hCG at 100,000 mIU/mL, hemoglobin at 0.1 mg/mL, lipids at 10 μg/mL, LH at 1,000 mIU/mL, PRL at 5,000 ng/mL, respectively
• Accuracy: r= 0.997 against reference ELISA by an external lab
• Intra Assay Precision: CV ≤ 6.4%
• Inter Assay Precision: CV ≤ 9.3%
• Hook Effect: No hook effect up to 10,000 ng/mL
• Shelf life: 18 months
• Open kit stability:  8 weeks at 2-8°C, or until the expiration date

 

The CTK Ferritin ELISA is a solid-phase enzyme-linked immunosorbent assay. The five components are:

• Solid microwells pre-coated with streptavidin
• FTN Calibrators
• FTN Assay Control
• FTN Biotin Reagent comprised of anti-FTN conjugated to biotin (Bio-Ab) and
• FTN Enzyme Reagent comprised of anti-FTN conjugated to HRP enzyme (HRP-Ab)

This test is intended for the quantitative determination of ferritin (FTN) concentration in human serum. It is intended to be used by professionals as an aid in the measurement of iron status. Any interpretation or use of this test result must also rely on other clinical findings as well as on the professional judgment of health care providers.

Recommended for use:

• Aid in measurement of iron status in human serum from individual subjects
• Monitor iron levels, especially if a patient has iron-related conditions such as anemia, hemochromatosis, porphyria, chronic inflammatory disorder, liver disease, hyperthyroidism, Leukemia, Hodgkin’s lymphoma, or alcohol abuse.

Ferritin is a 24-subunit, intracellular protein core that functions to store and release iron. Under normal circumstances, serum ferritin concentration is positively correlated with the total amount of iron stored in the body. Low concentration of serum ferritin usually indicates iron deficiency. Iron deficiency is one of the major causes of anemia which is most prevalent in highly susceptible populations, which include pregnant women and children. If serum ferritin concentration is high, it may indicate iron overload, and may be the result of high body mass index, aging, or the presence of inflammation, infection, or liver or kidney disease. Measurement of iron status by serum ferritin is important for accurate classification of iron status in individuals, in order to provide treatment for iron deficiency or plan of care for elevated serum ferritin concentration.