[ElisaKits] B-CELL ACTIVATING FACTOR (BAFF) ELISA KIT

[ElisaKits] B-CELL ACTIVATING FACTOR (BAFF) ELISA KIT

 

 

INTENDED USE

Human BAFF ELISA kit is designed to be used for measuring in vitro quantitative concentrations of B-cell activating factor (BAFF) in human cell culture supernatant, serum and plasma. This assay has a minimum detection limit of < 2.0 pg/ml.

BACKGROUND

B-cell activating factor (BAFF) which is also known as APOL-related leukocyte expressed ligand (TALL-1), dendritic cell-derived TNF-like molecule (CD257 antigen) and B Lymphocyte Stimulator (BLyS), is part of the tumor necrosis factor ligand superfamily member 13B proteins. BAFF is a glycoprotein which is 285 amino acid long at it has the ability to undergo glycosylation at residue 124. It is usually expressed as a membrane-bound type II transmembrane protein that is found on a number of different cells such as dendritic cells, bone marrow stromal cells and monocytes.

The following human BAFF ELISA kit is designed to be used for various types of diagnostic applications. BAFF is the natural ligand for three tumor necrosis factor receptors which are called BAFF-R (BR3), BCMA, and TACI and cyclophilin ligand interactor), all of these are displaying differing binding affinities for it. Signalling through BAFF-R or BCMA can be used to stimulate B lymphocytes to order to undergo proliferation and to counter apoptosis. Whereas, BCMA displays an intermediate binding phenotype and will work with APRIL and BAFF. BAFF is a cytokine which is a member of the tumor necrosis factor (TNF) ligand family and is a ligand for receptors TNFRSF13B/TACI, TNFRSF13C/BAFF-R and TNFRSF17/BCMA. It is essentially expressed in B cell lineage cells which can act as a potent B cell activators, it also displays vital roles in B cell proliferation and differentiation. Excessive level of BAFF have be identified to result in abnormally high antibody production which can cause rheumatoid arthritis, systemic lupus erythmatosis and a number of other autoimmune diseases. The standard that is used in this BAFF ELISA kit is a recombinant soluble human BAFF (A134-L295) that has a molecular mass of 19.6KDa.

SENSITIVITY

The minimum detection sensitivity level of B-cell activating factor (BAFF) using current human BAFF ELISA kit was 2.0 pg/ml. The dynamic range for this assay is 62.5 – 4,000 pg/ml.