[Cellomics Technology] Lentiviral BAP1 sgRNA CRISPR/Cas9

[Cellomics Technology] Lentiviral BAP1 sgRNA CRISPR/Cas9

 

GT-10002-50

Lentiviral BAP1 sgRNA CRISPR/Cas9 (1x10^7 TU/ml)

2x25 ul

GT-10002-200

Lentiviral BAP1 sgRNA CRISPR/Cas9 (1x10^7 TU/ml)

8x25 ul

 

Virus:	Lentiviral BAP1 sgRNA CRISPR/Cas9
Titer (approx):	1 x 107 TU/ml
Vector Information:	Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Promoter for sgRNA:	U6
Promoter for Cas9 nuclease:	Ef1a
Species:	Human
sgRNA target gene:	BAP1
sgRNA Sequence:	G AAC TCA GCG TCC CGG CCT G
Selection Gene:	Puromycin
Biosafety Level:	BSL-2
Shipped:	Dry ice
Storage:	Store at -80°C

 

BAP1 (BRCA1 Associated Protein 1) is a deubiquitylase thought to be a key regulator of many cancer-associated pathways. BAP1 binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. 

This lentivirus expresses human BAP1 sgRNA under the control of the U6 promoter, Cas9 nuclease and puromycin under the control of the EF1a promoter.