[Cellomics Technology] Lentiviral vector-CAG, Hyg
|
LVR-1021
|
pLV-CAG-MCS-PGK-Hyg
|
10 µg |
| Vector: | pLV-CAG-MCS-PGK-Hyg |
| DNA amount | 10 µg |
| Vector Information: | Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) |
| Promoter for Target Gene: | CAG |
| Selection Gene: | Hyg |
| Promoter for Selection Gene | PGK |
| Biosafety Level: | BSL-2 |
| Shipped: | Room Temperature |
| Storage: | Store at -20°C |
- High Protein Expression Levels
- Extremely Low recombination rate
- Compatible with 2nd and 3rd generation of packaging systems, ideal with EZ-LentiPACK Packaging System
Lentiviral vector-CAG, Hyg contains Multiple Cloning Sites (MCS) downstream of the CAG promoter, and hygromycin driven by the PGK promoter as a selection marker. Clone your gene of interest into the MCS, then co-transfect with EZ-LentiPACK Packaging System into packaging cells, such as HEK293T to produce VSV-G pseudotyped lentivirus. Transduced stable cells can be selected by hygromycin.
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