[Signalway Antibody - SAB] Sequencing Grade V8 Protease #RE014
- Product Name
- Sequencing Grade V8 Protease
- Purification
- Purity(SDS-PAGE): Single major band
- Species Reactivity
- 5.0 AU /mg pro.
- Target Name
- Sequencing grade recombinant V8 protease;Staphylococcus aureus strain V8
- Alternative Names
- CAS No.
- 66676-43-5
EC:3.4.21.19 - SDS-PAGE MW
- 24.02.4 kDa
- Storage
- Stability of storage: Recombinant V8 protease should be stored under 2-8C in sealed container.It is stable within 6 months after dissolved with 1mM HCl and 50mM HAC at -20C or below. It is stable within 2 months after dissolved with 1mM HCl and 50mM HAC at 4C.85% activity above can be kept after dissolved with 1mM HCl and 50mM HAC at 25C or 37C 12h. It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
- Recombinant V8 protease is the serine protease family and can specifically hydrolyzing glutamic acid or aspartic acid residues carboxy side peptide bonds. The Glu carboxy terminal peptide bond can be identified and cleaved in CH4COONH4 buffer (pH 7.8) and NH4HCO3 (pH 4.0 ).Identify and cleave the Glu or Asp carboxy terminal peptide bond in phosphate buffer (pH 7.8),and the hydrolysis rate of Glu was higher than that of Glu Asp. Recombinant V8 protease has activity between pH 4.0-10.0, and the optimum pH is 8.0-8.5. It can be used alone or in combination with other proteases when used in protein digestion, peptide profiling and peptide mass fingerprinting analysis. The inhibitors of V8 protease are diisopropylfluorophosphate (DFP), α2-macroglobulin and Nα-P-tosyl-L-lysine chloromethyl ketone (TLCK).
