[Leading Biology] Anti-M13 Bacteriophage polyclonal antibody(HRP)

[Leading Biology] Anti-M13 Bacteriophage polyclonal antibody(HRP)

 

 

 

AH0013R1 / 100ul

Brand	Leading Biology	Catalog Number	AH0013R1
Product Type	Secondary Antibody	Field of Research

Product Overview	M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic nor temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.
Host	Rabbit
Immunogen	M13 Bacteriophage
Conjugation	HRP
Form	Liquid
Purity	Purification: Protein A
Storage & Stability	stored at -20°C to -80°C , Avoid repeated freeze-thaw cycle
Storage Buffer	PBS (pH=7.40)
Dilution	ELISA: 1:3000~1:5000
Product Description	Antibody notes： Can be used as a reagent in “phage ELISA” offering sensitive and specific activity for detection of recombinant phages.
References	Messing, J . et al., (1993). Methods in Molecular Biology. 23. pp. 9–22. Phage Display: A Laboratory Manual Sitohy, M. et al., （2006）. J Agric Food Chem. 54 (11): 3800-6. Phipps ML et al. , (2016). PLoS One11:e0160940