[FenicsBio] EF1a-Cas9 lentivirus (Hyg)
LV-1270-25
EF1a-Cas9 lentivirus (Hyg)
25 ul (1x25ul)
LV-1270-100
EF1a-Cas9 lentivirus (Hyg)
100 ul (4x25ul)
| Package Size | |
| Functional titer | 1x10^8 TU/ml |
| Titration method | counting Hygromycin resistant cell clones |
| Purity | In vivo grade |
| Pseudotype | VSV-G |
| Target Gene | eSpCas9 |
| Promoter for target gene | EF1a |
| Mammalian selection | Hygromycin |
| Promoter for selection gene | PGK |
| Storage Buffer | LentiShield™Lentivirus storage buffer |
| Storage | -80°C |
| Shipping condition | Dry ice |
Catalog number
LV-1270
Description
Cas9 is a protein which plays a vital role in the immunological defense of certain bacteria against DNA viruses. Cas9 has been widely used for gene editing in mammalian cells. eSpCas9 (enhanced specificity Cas9) has enhanced specificity compared to wild type Cas9 for CRISPR/Cas9 genome editing. eSpCas9 is able to reduce off-target effects and maintain robust on-target genome editing efficiency, allowing more efficient gene targeting. EF1a-Cas9 lentivirus (Hyg) expresses eSpCas9 under the control of the EF1a promoter with PGK promoter diven hygromycin as a selection marker, allowing selecting stable cell clones with hygromycin.
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