[FenicsBio] Lentiviral GFP-miR-shRNA cloning vector (tet, Puro)

[FenicsBio] Lentiviral GFP-miR-shRNA cloning vector (tet, Puro)

 

Catalog number

AB1059

 

Vector name	pLV-tet-GFP-miR-PGK-Puro
Package Size	10 ug
E.coli selection	Amp
Promoter for target gene	TRE
Mammalian selection	Puromycin
Promoter for selection gene	PGK
Storage	-20°C

Features:

• A third generation lentiviral vector
• Extremely Low recombination rate
• Compatible with 2nd and 3rd generation of packaging systems, ideal with our Lentivirus Packaging System.

 

Lentiviral GFP-miR-shRNA cloning vector (tet, Puro) is a third generation lentiviral vector, with tetracycline-responsive promoter element (TRE) to drive GFP and miR-shRNA coexpression and PGK promoter to drive selection marker puromycin expression. This vector is compatible with both 2nd and 3rd generation packaging systems.

Clone your miR-shRNA into the Lentiviral GFP-miR-shRNA cloning vector (tet, Puro), then co-transfect with selected Lentivirus Packaging System into packaging cells, such as HEK293T cells to produce lentivirus. Lentivirus transduced cells can be selected by puromycin to generate stable cell lines.