[FenicsBio] AAV Production

[FenicsBio] AAV Production

 

 

 

 

 

 

 

C-AAV-11

AAV production-Small scale

 

C-AAV-12

AAV production-Standard scale

 

C-AAV-13

AAV production-Large scale

 

Features:

• One-stop cloning service, can start from scratch
• Fast turnaround, turnaround in 2-3 weeks
• Project update weekly, and Q/C reports in every milestone.

Deliverables/Lead Time

SKU No.	Production scale	Deliverables	Purity grade	Titration	Lead time
C-AAV-11	>1x10^11 GC/ml, 200ul	AAV particles, 4x50ul	In vitro	Real-time PCR	2-3 weeks
C-AAV-12	>1x10^12 GC/ml, 1ml	AAV particles, 5x200ul	In vitro	Real-time PCR	2-3 weeks
C-AAV-13	>1x10^13 GC/ml, 500ul	AAV particles, 5x100ul	In vitro and In vivo	Real-time PCR	2-3 weeks

Note: If construct generation is required, another 1-2 weeks are needed for most constructs.

QC Testing

The Q/C testing for FenicsBIO’s Custom AAV Production Service includes:

• Restriction digestion pattern verification and sequence confirmation for the AAV constructs if generated by FenicsBIO
• AAV viral particles sterility testing, titration by Real-time PCR

Project Initiation

Phase I: Project information collection

Our scientists will work with you to figure out the details of your AAV production requirement, including AAV titer, volume and the serotype, whether you need us to generate the AAV constructs and if you do, what requirements you have for the constructs, including the promoter to use, selection marker and reporter requirements.

Phase II: Project plan approval

Based on the above information you provided, we will formulate a project plan and send you a quotation. After you approve the project plan, you can use the quotation to place an order.

Phase III: Starting material shipment

If you have AAV construct, please send at least 1 ug of the plasmid, bacterial stab or glycerol stock. In most cases, these starting materials can be shipped at room temperature.

If you need us to generate AAV constructs, your project manager will send you a list of starting material needed.

 

 

Adeno-associated virus (AAV) vector is among the most commonly used vectors for in vivo gene therapy. AAV vector stands out by its safety profile. Infection of AAV vector is not pathogenic, it does not integrate into the host genome and instead forms episomal concatemers in the cell nucleus, therefore will not generate integration related mutagenesis.  AAV vectors demonstrate high transduction efficiency to both dividing and non-dividing cells, stable long-term expression, low immunogenicity and selective tissue tropism.

FenicsBIO provides superior quality, fast turnaround AAV production service to meet your AAV-based research needs. We have developed proprietary reagents and technologies to cover every step of AAV production, including construct generation, AAV packaging, purification and storage.