[Leading Biology] Functional APRIL (mouse) Antibody, mAb (recombinant) (blocking) (Biotin)

[Leading Biology] Functional APRIL (mouse) Antibody, mAb (recombinant) (blocking) (Biotin)

 

 

 

 

AMP00003G

Brand	Leading Biology	Catalog Number	AMP00003G
Product Type	Monoclonal Antibodies	Field of Research

Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Functional APRIL (mouse) Antibody, mAb (recombinant) (blocking) (Biotin).
Molecular Weight	26889 Da
Cellular Localization	Antigen Cellular Localization: Secreted.
Host	Purified From?HEK 293 Cell?culture Supernatant.
Species Reactivity	Mouse
Target	Recognizes mouse APRIL. Does not recognize mouse BAFF.
Clone	Apry-1-1
Isotype	Mouse IgG2bλ
Symbol	April
GeneID	69583
UniProt ID	Q9D777
Function	Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
Summary	APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth and is involved in monocyte/macrophage-mediated immunological processes.Anti-APRIL (mouse) Monoclonal Antibody (recombinant) (Blocking) (APRY-1-1) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Form	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	E, IP
Images	Binding of APRIL (mouse) to BCMA is inhibited by anti-APRIL (mouse), mAb (rec.) (blocking) (APRY-1-1).BCMA:Fc was coated on an ELISA plate at 1ug /ml. anti-APRIL (mouse) mAb (rec.) (blocking) (APRY-1-1) or an unrelated mAb (recombinant) (Negative control) were added (starting at 50ug/ml with a twofold serial dilution) together with 0.1ug /ml of MegaAPRIL (mouse). After incubation for 1 h at RT, the MegaAPRIL (mouse) binding was detected using an anti-FLAG antibody (HRP). The percentage of binding is shown.