[Signalway Antibody] GFP Antibody
35538
Description
- Product Name
- GFP Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Purification
- Antigen affinity purification.
- Applications
- WB
- Specificity
- The antibody detects transfected proteins contanining GFP tag.
- Immunogen Type
- Recombinant Protein
- Immunogen Description
- Full length fusion protein
- Target Name
- GFP
- Alternative Names
- GFP-like chromoprotein
- Accession No.
- Gene Accssion: P42212
- SDS-PAGE MW
- 27kd
- Concentration
- 1.6mg/ml
- Formulation
- Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
- Storage
- Store at -20˚C
Application Details
Western blotting: 1:1000-1:10000
Gel: 8%SDS-PAGE
Lysate: 20ug GFP transfected 293T cell
Primary antibody: 1/800 dilution
Secondary antibody dilution: 1/8000
Exposure time: 30 seconds
Gel: 8%SDS-PAGE
Lysates: 20ug GFP transfected 293T cell per lane
Primary antibody: at various dilution
Secondary antibody dilution: 1/8000
Exposure time: 30 seconds
Background
Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.
