[Creative Biogene] Wheat CRISPR/Cas9 Genome Editing Kit (Basta)

[Creative Biogene] Wheat CRISPR/Cas9 Genome Editing Kit (Basta)

 

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Cat.No.

CC-11

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Description

The Wheat CRISPR/Cas9 Genome Editing Kit is a highly effective and universal gene editing kit. It is intended for Agrobacterium-mediated plant transformation. The CRISPR construct contains a sgRNA expression cassette driven by TaU6 promoter, and a codon optimized Cas9 expression cassette driven by ubiquitin promoter. The Wheat CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in wheat.

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Applications

The Wheat CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in wheat.

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Efficiency

Cas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.

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Features

•Two functional components are combined into one vector which offers a higher transfection efficiency.

•High expression level of Cas9 protein in monocots by using maize ubiquitin promoter and monocot codon optimized-Cas9.

•High activity of sgRNA in rice by using TaU6 (wheat U6) promoter.

•Mutiplex genome editing

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Resistance

Kanamycin

Selection Marker

Basta

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