[TaiClone] Recombinant Rabbit Anti-Human IgG4 Antibody

[TaiClone] Recombinant Rabbit Anti-Human IgG4 Antibody

 

 

 

 

 

 

 

 

tcna4771

 

 

Form

Liquid

 

Storage Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

 

Recommended Dilution

 

ELISA: 50ng/well – 200ng/well (Capture)
0.05-0.2ug/ml (Detection)
Western Blot: 0.5-2ug/ml
Immunocytochemistry: 1-10ug/ml
Immunohistochemistry: 1-10ug/ml (1)The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

 

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

 

Application

WB, ICC, IHC, FACS, ELISA

 

Species Reactivity

Human

 

Host Species

Rabbit

 

Immunogen / Amino acids

Peptide corresponding to the hinge region of human IgG4 was used as the immunogen for this recombinant Human IgG4 antibody.

 

Conjugation

Biotin Conjugate

 

Clonality

Recombinant Rabbit Monoclonal

 

Clones

RM120

 

Isotype

Rabbit IgG