[AddexBio] MG-63 cells

[AddexBio] MG-63 cells

 

Catalog #:

C0004007

Disease:

Osteosarcoma

Origin:

From an osteosarcoma of a 14 year old male. Produces high yields of interferon following superinduction with polyinosinic acid-polycytidilic acid, cyclohexamide and actinomycin. Antigenically, MG-63 interferon is more closely related to human fibroblast than to leukocyte interferon.

Species:

Homo sapiens, human

Tissue:

Bone

Morphology:

Fibroblast

Properties:

Adherent

Cytogenic data:

This is a hypotriploid human cell line. The modal chromosome number was 66 occurring in 44% of cells. The rate of cells with higher ploidies was 2.0%. Eighteen to 19 marker chromosomes were common to all cells.

Patient:

Caucasian, Male, 14 years of age

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Subculture at a ratio of 1:4 to 1:8 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete growth culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:

I

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:

HIV: Negative

Hepatitis B: Negative

DNA Profile (STR):

Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 11

D16S539: 11

D5S818: 11,12

D7S820: 10

THO1:9.3

TPOX: 8,11

vWA: 16,19

Citation Format:

If use of this culture results in a scientific publication, it should be cited in the publication as: MG-63 (AddexBio C0004007)

References for MG-63 cells:

1. Billiau A, Edy VG, Heremans H, Van Damme J, Desmyter J, Georgiades JA, De Somer P. Human interferon: mass production in a newly established cell line, MG-63. Antimicrob Agents Chemother. 1977;12(1):11-15. PMID: 883813