[AddexBio] A-172 cells

[AddexBio] A-172 cells

 

Catalog #:

C0005009

 

Disease:

Glioblastoma

Origin:

Derived from a glioblastoma removed from a 53 year old male. Non-tumourigenic in anti-thymocyte serum treated NIH Swiss mice.

Species:

Homo sapiens, human

Tissue:

Brain

Morphology:

Epithelial-like with processes and/or rounded

Properties:

Adherent

Cytogenic data:

n = 80, over 20 marker chromosomes per cell (hypertriploid)

Patient:

Male, 53 years of age

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:3 to 1:8 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO

Biosafety Level:

I

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:

HIV: Negative

Hepatitis B: Negative

DNA Profile (STR):

Amelogenin: X,Y

CSF1PO: 9,12

D13S317: 11

D16S539: 12

D5S818:11,12

D7S820: 11

THO1: 6, 9.3

TPOX: 8,11

vWA: 20

Citation Format:

If use of this culture results in a scientific publication, it should be cited in the publication as: A-172 (AddexBio C0005009)