[Cellomics Technology] Lentiviral vector-CAG, Hyg

[Cellomics Technology] Lentiviral vector-CAG, Hyg

 

LVR-1021

pLV-CAG-MCS-PGK-Hyg

10 µg

 

Vector:	pLV-CAG-MCS-PGK-Hyg
DNA amount	10 µg
Vector Information:	Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Promoter for Target Gene:	CAG
Selection Gene:	Hyg
Promoter for Selection Gene	PGK
Biosafety Level:	BSL-2
Shipped:	Room Temperature
Storage:	Store at -20°C

 

Lentiviral vector-CAG, Hyg contains  Multiple Cloning Sites (MCS) downstream of the CAG promoter, and hygromycin driven by the PGK promoter as a selection marker. Clone your gene of interest into the MCS, then co-transfect with EZ-LentiPACK Packaging System into packaging cells, such as HEK293T to produce VSV-G pseudotyped lentivirus. Transduced stable cells can be selected by hygromycin.

• High Protein Expression Levels
• Extremely Low recombination rate
• Compatible with 2nd and 3rd generation of packaging systems, ideal with EZ-LentiPACK Packaging System