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Hawach Scientific

[Hawach Scientific] C18-Aqueous HPLC Columns

by 휴바이오랩(Hubiolab) 2024. 9. 9.

[Hawach Scientific]  C18-Aqueous HPLC Columns

 

Column: XChroma HPLC Column, C18-Aqueous, 5μm, 4.0×150mm
Mobile Phase: Methanol/acetonitrile/water=38/38/24
Wavelength: 245nm
Temperature: 30°C
Flow Rate: 1mL/min
Injection Volume: 5μL
 
SLHC18A32130 HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x30mm 1
SLHC18A32133 HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x33mm 1
SLHC18A32150 HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x50mm 1
SLHC18A32175 HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x75mm 1
SLHC18A321100 HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x100mm 1
SLHC18A321125 HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x125mm 1
SLHC18A321150 HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x150mm 1
SLHC18A321200 HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x200mm 1
SLHC18A321250 HPLC Column, C18-Aqueous, 3μm, 120Å, 2.1x250mm 1
SLHC18A33030 HPLC Column, C18-Aqueous, 3μm, 120Å, 3.0x30mm 1
SLHC18A33050 HPLC Column, C18-Aqueous, 3μm, 120Å, 3.0x50mm 1
SLHC18A33075 HPLC Column, C18-Aqueous, 3μm, 120Å, 3.0x75mm 1

 

Advantage of C18 Aqueous HPLC columns

  • The most widely applied C18-Aqueous HPLC column in the analysis of the food industry;
  • 300 Å pore size available for the separation of macromolecular compounds;
  • Highly specific surface area and mechanical strength ensured from high purity silica gel;
  • Double end-capped for excellent peak shape when separation of polar, acidic, and basic compounds;
  • Column peak shape and volume are perfect by spherical silica gel and good stability of batch to batch;
  • Buffer systems and ion pair reagents do not need for the analysis of drugs, combined chemicals, and LC-MS applications.

Features

  • Increase column retention for polar and water-soluble compounds;
  • The fully end-capped stationary phase offers a longer column lifetime;
  • Less retentive than C18 Universal for non-polar compounds;
  • No phase collapse and especially suitable for high aqueous-based mobile phase;
  • Excellent column peak shapes for basic and polar compounds are ensured by the fully end-capped stationary phase.
Bonded Phase USP No. Particle Size Pore Size Surface Area(m2) Carbon Loading pH range End-capped
C18-aqueous L1 3 μm, 5 μm, 10 μm 120Å 320 12% 1.5-10 Yes

Application

  • Separation of metabolites and bio-molecules;
  • Excellent column efficiency especially for applications of water-soluble compounds which cannot be retained on the traditional C18 stationary phase;
  • Drug degradation products Separation such as water-soluble vitamins, small molecules, amino acids, and small molecules of the nucleoside.

 

Descriptions of C18-Aqueous Liquid Chromatography Column

C18-Aqueous HPLC Column can endure 100% aqueous phase samples. With appropriate surface coverage and double end-capped technique, the C18-Aqueous liquid chromatography column has good compatibility with highly aqueous-based mobile phase and can provide a longer column lifetime. The stationary phase was specially made under the double end-capped technique making it stable and specially used for the aqueous-based mobile phase.