[Creative Biogene] MIRLET7e-pCMV-MIR

[Creative Biogene]  MIRLET7e-pCMV-MIR

 

Cat.No.

MIPH2007

Description

Expression plasmid for human microRNA LET7e. All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA. Both mature and minor (star) miRNA can be over expressed from the construct.

Reporter

GFP

Size/Form

The plasmid is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5 and 3 vector primers in separate vials.

Species

Human

Vector Type

plasmid

miRNA Information

Precursor Accession No.

MI0000066

Precursor Name

hsa-let-7e

Precursors sequence

CCCGGGCUGAGGUAGGAGGUUGUAUAGUUGAGGAG GACACCCAAGGAGAUCACUAUACGGCCUCCUAGCU UUCCCCAGG

Precursors Length

79 bp

Gene Description

Homo sapiens let-7e stem-loop

Gene Species

Human

Mature miRNA-1 Accession No.

MIMAT0000066

Mature miRNA-1 Name

hsa-let-7e-5p

Mature miRNA-1 sequence

UGAGGUAGGAGGUUGUAUAGUU

Mature miRNA-2 Accession No.

MIMAT0004485

Mature miRNA-2 Name

hsa-let-7e-3p

Mature miRNA-2 sequence

CUAUACGGCCUCCUAGCUUUCC