[Creative Biogene] T4 Polynucleotide Kinase

[Creative Biogene] T4 Polynucleotide Kinase

Cat.No.

EMQZ1405

 

 

Description

Catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates. Polynucleotide Kinase also catalyzes the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates.

 

Applications

• End-labeling DNA or RNA for probes and DNA sequencing

• Addition of 5´-phosphates to oligonucleotides to allow subsequent ligation• Removal of 3´-phosphoryl groups

 

Concentration

10,000U/ml

 

Reaction Conditions

1×T4 Polynucleotide Kinase Reaction System containing: 1×T4 Polynucleotide Kinase Reaction Buffer [70 mM Tris-HCl (pH 7.6 at 25°C),10 mM MgCl2,5 mM DTT]; incubate at 37°C.

 

Size/Form

200U; 1,000U

 

Source

A recombinant E. coli strain that carries the cloned T4 Polynucleotide Kinase gene.

 

Storage

Store at -20°C

 

Unit Definition

One Richardson unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of acid-insoluble [32P] in 30 minutes at 37°C.