[Raybiotech] Human Cytokine Array C7

[Raybiotech] Human Cytokine Array C7

 

CODE: AAH-CYT-7-2

 

Antigen Information

Gene Symbols:

ADIPOQ | AGRP | ANGPT2 | AREG | AXL | BTC | CCL16 | CCL19 | CCL25 | CCL27| CCL28 | CCL3 | CCL4 | CSF3 | CXCL1 | CXCL11 | CXCL2 | CXCL3 | CXCL5 | CXCL8 | EGFR | FAS | FGF2 | FGF4 | FGF9 | FIGF | HGF | ICAM1 | ICAM3 | IGF1R | IGFBP3 | IGFBP6 | IL11 | IL12A | IL12B | IL17A | IL1R1 | IL1RL1 | IL2RA | IL6R | IL6ST | MIF | MST1 | NGF | NTF4 | OSM | PGF | PLAUR | THPO | TIMP1 | TIMP2 | TNFRSF10C | TNFRSF10D | TNFRSF11B | TNFRSF18 | TNFRSF1A | TNFRSF1B | TNFSF18 | TPO | TYRO3 | VEGFA | XCL1   Hide

Assay Format

Number of Targets Detected:

60

Species Detected:

• Human

Compatible Sample Types:

• Cell Culture Supernatants
• Cell Lysates
• Plasma
• Serum
• Tissue Lysates

Design Principle:

• Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Product Features

• Easy to use
• No specialized equipment needed
• Compatible with nearly any liquid sample
• Proven technology (many publications)
• Highly sensitive (pg/ml)
• Sandwich ELISA specificity
• Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

Adiponectin (ACRP30)	AgRP	Angiopoietin-2	Amphiregulin	Axl
bFGF	beta-NGF	Betacellulin (BTC)	CCL28 (MEC)	CTACK (CCL27)
Dtk	EGFR	ENA-78 (CXCL5)	Fas (TNFRSF6/Apo-1)	FGF-4
FGF-9	GCSF	GITR Ligand (TNFSF18)	GITR (TNFRSF18)	GRO alpha/beta/gamma
GRO alpha (CXCL1)	HCC-4 (CCL16)	HGF	ICAM-1 (CD54)	ICAM-3 (CD50)
IGFBP-3	IGFBP-6	IGF-1 R	IL-1 R4 (ST2)	IL-1 R1
IL-11	IL-12 p40	IL-12 p70	IL-17A	IL-2 R alpha
IL-6 R	IL-8 (CXCL8)	I-TAC (CXCL11)	Lymphotactin (XCL1)	MIF
MIP-1 alpha (CCL3)	MIP-1 beta (CCL4)	MIP-3 beta (CCL19)	MSP alpha/beta	NT-4
Osteoprotegerin (TNFRSF11B)	Oncostatin M	PLGF	gp130	TNF RII (TNFRSF1B)
TNF RI (TNFRSF1A)	TECK (CCL25)	TIMP-1	TIMP-2	Thrombopoietin (TPO)
TRAIL R3 (TNFRSF10C)	TRAIL R4 (TNFRSF10D)	uPAR	VEGF-A	VEGF-D

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

• Human Cytokine Antibody Array C7 Membranes
• Blocking Buffer
• Wash Buffer 1
• Wash Buffer 2
• Biotinylated Detection Antibody Cocktail
• Streptavidin-Conjugated HRP
• Detection Buffer C
• Detection Buffer D
• Lysis Buffer
• 8-Well Incubation Tray
• Plastic Sheets
• Array Templates
• Manual

Other Materials Required

• Pipettors, pipet tips and other common lab consumables
• Orbital shaker or oscillating rocker
• Tissue Paper, blotting paper or chromatography paper
• Adhesive tape or Saran Wrap
• Distilled or de-ionized water
• A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

1. Block membranes
2. Incubate with Sample
3. Incubate with Biotinylated Detection Antibody Cocktail
4. Incubate with HRP-Conjugated Streptavidin
5. Incubate with Detection Buffers
6. Image with chemiluminescent imaging system
7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.