[Kamiya Biomedical] Caspase-2 Enzyme (human, recombinant)
Cat. No: BC-118 (25 U)
BC-119 (100 U)
Description: Caspase-2 (also known as Ich-1,
Nedd-2) is a member of the interleukin-1
converting enzyme (ICE) family of cysteine
proteases. Similar as other caspases, caspase-2
also exists in cells as an inactive proenzyme.
During apoptosis, procaspase-2 is processed at
aspartate residues by self-proteolysis and/or
cleavage by upstream caspases. The processed
form of caspase-2 consists of large (19 kDa) and
small (12 kDa) subunits, which associate to form
the active enzyme. The active recombinant
human caspase-2 was expressed in E. coli. The
expressed caspase-2 spontaneously undergoes
auto-processing to yield the subunits
characteristic of the native enzyme.
Origin: E. coli
Format: Lyophilized powder.
Reconstitution:
Reconstitute with PBS to 1 unit/µl.
Specific Activity: ~10,000 units/mg. One unit
cleaves 1nmole of the caspase substrate
VDVAD-pNA per hour at 37°C in a reaction
solution containing 50mM HEPES, pH 7.2,
50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5%
glycerol and 10mM DTT.
Figure: Active human caspase was expressed in
E. coli and purified. The activity of recombinant
caspase-2 was determined by cleaving AFC
conjugates of VDVAD. The cleavage activity
was effectively inhibited by the corresponding
peptide inhibitor as indicated.
Storage and Stability:
Store at -80°C. Avoid freeze / thaw cycles. After
reconstitution, prepare aliquots and store at
-80°C.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.
