[Leading Biology] Purified anti-Podoplanin (Lymphatic Endothelial Marker) Antibody
LM13562T
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Brand
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Leading Biology
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Catalog Number
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LM13562T
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Product Type
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Primary Antibody
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Field of Research
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Product Overview
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Podoplanin is expressed by a variety of normal cells, which include breast and prostate myopepithelial cells, follicular dendritic cells, basal keratinocytes of the skin and cervix (focal), type I pneumocytes, ependymal cells, and fetal cerebral germinal matrix cells. Podoplanin is a marker of lymphatic differentiation because it is expressed by normal human lymphatic endothelium, but not by vascular endothelium. It is diagnostically a useful marker for the evaluation of a variety of neoplasms, including Kaposi sarcoma mesothelioma, testicular germ cell tumors, and cutanneous sebaceous neoplasms.
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Host
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Mouse
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Species Reactivity
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Human
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Immunogen
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M2A protein derived from human germ cell tumors.
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Clone
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D2-40
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Isotype
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Mouse IgG1, κ
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Concentration
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1.0 mg/ml
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Preparation
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The antibody was purified by affinity chromatography.
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Storage & Stability
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The antibody solution should be stored undiluted between 2°C and 8°C.
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Applications
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IHC-P WB ELISA Detection ICC
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Product Description
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Podoplanin is expressed by a variety of normal cells, which include breast and prostate myopepithelial cells, follicular dendritic cells, basal keratinocytes of the skin and cervix (focal), type I pneumocytes, ependymal cells, and fetal cerebral germinal matrix cells. Podoplanin is a marker of lymphatic differentiation because it is expressed by normal human lymphatic endothelium, but not by vascular endothelium. It is diagnostically a useful marker for the evaluation of a variety of neoplasms, including Kaposi sarcoma mesothelioma, testicular germ cell tumors, and cutanneous sebaceous neoplasms.
IHC staining of purified anti-Podoplanin (Lymphatic Endothelial Marker) antibody (clone D2-40) on formalin-fixed paraffin-embedded human colon tissue. The tissue was incubated with 1.3 µg/ml of the primary antibody at room temperature for 60 minutes. BioLegend's Ultra-Streptavidin (USA) HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
M2A protein derived from human germ cell tumors.
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application
IHC-P
WB
ELISA Detection, ICC
Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.5 - 5.0 μg/ml is suggested. For Western blotting, the suggested use of this reagent is 2.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes
Additional reported applications (for the relevant formats) include:
ELISA Detection and immunocytochemistry.
Structure
35 kD.
Distribution
Membrane and plasma membrane.
Function
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels.
Interaction
Cytoskeletal signaling.
Biology Area
Cancer Biomarkers, Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Gene ID
UniProt
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hubiolab@gmail.com
010-9468-7169
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