[HepatoChem] Suzuki Cross Coupling Array: Standard Kit Aqueous Base
(HCK1003-01-001)
Protocol at 100 µl volume reaction condition
- Prepare required volume of a 0.15 mol/L solution of combined substrates (boronic acid reagent and halogenated substrate) in the selected solvent. For example, 450 µl solution for 4 reaction conditions.
- Using a clean and dry syringe, add 100 µl of substrates solution to a reaction vial and mix with the catalysts using the syringe.
- Add 30 µl of the selected base solution. (2 equivalents)
- Stir the reaction vials in the reaction block at desired temperature (for example: 80°C) for 3 to 12 hours.
- Remove the vial caps using a decapper.
- Prepare analytical sample for each reaction condition with 5 µl sample diluted into 200 µl in either DMSO or water/acetonitrile 50/50. Analyze resulting analytical samples by LC/MS.
1 set of 6 catalysts (Pd(PPh3)4, Pd(dppf)2Cl2, PdOAc2/Sphos, PdOAc2/Xphos, Pd2(dba)3/Sphos, and Pd2(dba)3/Xphos), 4 base solutions (Na2CO3, K2CO3, K3PO4, and Cs2CO3) and 4 solvents Acetonitrile, Dioxane, DMF, and n-Butanol. 24 reaction conditions
This kit requires EvoluChem™ reaction block (HCK1006-01-002) included in the EvoluChem™ Starter Kit (HCK1006-01-001)
The typical protocol is performed at 0.15 mol/l concentration reaction condition and 1 to 1 ratio boronic acid reagent and halogenated substrate in 100 µl selected solvent. Resulting catalyst loading is 10%. Two equivalents of base are added (30 µl). However it is possible to investigate different substrate ratios and concentrations. Maximum volume for reaction condition is 250 µl.
