[Creative Biogene] Premix qRT Master Mix Kit

[Creative Biogene] Premix qRT Master Mix Kit

 

 

 

 

 

Cat.No.QBHC-02

 

Description

PrimeScript RT Master Mix (Perfect Real Time) is designed for fast, efficient reverse transcription prior to real-time RT-PCR (qPCR) with a streamlined workflow. cDNA synthesis reactions are easy to assemble – just add RNA template and water. This kit is designed for two-step real-time RT-PCR. Use it upstream of a SYBR or TaqMan qPCR premix.
This kit includes Reverse Transcriptase, which has exceptionally strong strand displacement activity for optimal efficiency during cDNA synthesis.

 

Applications

Real-Time PCR using either Taqman Probes or SYBR Green I Dye

 

Features

•Reverse transcription in just 15 minutes
•Master Mix format reduces pipetting steps and the possibility of contamination
•Works efficiently with GC-rich and/or higher-structured RNA templates
•Synthesizes full-length cDNA with high accuracy and at high yield prior to qPCR analysis

 

Method

Probe and SYBR Green I Dye based

 

Report Gene

Both SYBR Green and probe and two tube reaction

 

Sample Type

RNA

 

Selection Marker

DNA

 

Sensitivity

Variable

 

Storage

Store at -20°C and at dark condtion