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[Cellomics Technology] Lentiviral vector, synapsin 1 promoter, Hyg

by 휴바이오랩(Hubiolab) 2021. 8. 25.

[Cellomics Technology] Lentiviral vector, synapsin 1 promoter, Hyg

 

LVR-1074

 

Vector: pLV-hsyn1-MCS-PGK-Hyg
DNA amount 10 µg
Vector Information: Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Promoter for Target Gene: human synapsin 1 promoter
Selection Gene: Hygromycin
Promoter for Selection Gene PGK
Biosafety Level: BSL-2
Shipped: Room Temperature
Storage: Store at -20°C
  • High Protein Expression Levels
  • Extremely Low recombination rate
  • Compatible with 2nd and 3rd generation of packaging systems, ideal with EZ-LentiPACK Packaging System  

 

Human synapsin 1 promoter confers highly neuron-specific gene expression. Lentiviral vector-human synapsin 1 promoter, Hyg contains  Multiple Cloning Sites (MCS) downstream of the human synapsin 1 promoter, and a hygromycin selection marker driven by the PGK promoter.  Clone your gene of interest into the MCS, then co-transfect with EZ-LentiPACK Packaging System into packaging cells, such as 293T to produce VSV-G pseudotyped lentivirus. Transduced stable cells can be selected by hygromycin.

 

 

#LVR1074 #Lentiviralvector #