[HemaCare] Dendritic Cells

[HemaCare] Dendritic Cells

 

 

 

 

 

 

Peripheral Blood

Healthy

CryoStor® CS10

>90% by flow cytometry

>80% by flow cytometry

Storage Conditions:

 

For cryopreserved dendritic cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

 

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanosoma cruzi, and Syphilis.

 

Dendritic cells are highly specialized antigen-processing and antigen-presenting cells that are located in mucosal tissues, skin, and lymphoid tissues. They are also called accessory cells.

 

Dendritic cells play a major role in the initiation of immune responses as they act as messengers between the innate and adaptive immune systems. The main function of dendritic cells is to process and present antigens on the cell surface of T cells. In addition, dendritic cells contribute to the maintenance of B cell function and recall responses.

Dendritic cells have been used to develop in vitro assay methods for immunological reactions including T-cell stimulation, antigen presentation, contact sensitization, and more.

 

Dendritic cells are derived from purified monocytes. CD14 monocytes are positively selected and are cultured in the presence of GM-CSF and IL-4 for 7 days; and then harvested.