[Creative Bioarrray] ActoFactor™ Recombinant Human Interleukin 12 (chimera)
Cat.No.
CSC-CTK0014
Description
Human Interleukin 12 (IL-12) is a species specific cytokine produced by macrophages and B lymphocytes. IL-12 induces the production of IFN-g and TNF by resting and activated T and NK cells, synergizes with other IFN-g inducers at both the transcriptional and post-translational levels to induce IFN-g gene expression, enhances the cytotoxic activity of resting NK and T cells, induces and synergizes with IL-2 in the generation of lymphokine-activated killer (LAK) cells, and promotes proliferation of resting T cells and activated NK and T cells. IL-12 is a central mediator of cell-mediated immunity by its effect on development, proliferation and activation of Th1 cells. IL-12 is a glycosylated disulfide-linked heterodimeric protein, consisting of a 306 amino acid residue p40 subunit and a 197 amino acid residue p35 subunit; it has a molecular mass of approximately 75 kDa.
Organism
Human
Product Overview
Human IL12 (chimera) expressed in Sf21 insect cells
Sequence
DNA sequences encoding the p35 and p40 subunits of the human IL-12 protein were co-expressed in Sf21 insect cells.
Size
CAT# CSC-CTK0014-5 (5 μg); CAT# CSC-CTK0014-25 (25 μg)
Expression System
Sf21 insect cells
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
Activity was determined in a cell proliferation assay using PHA-activated human T lymphoblasts and the ED50 using this assay was typically 0.05 – 0.2 ng/mL.
Endotoxin Level
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Formulation
Lyophilized from a sterile-filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg ofcytokine.
Reconstitution
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 mg/mL of the cytokine.
Storage & Stability
The lyophilized sample is stable for greater than six months at -20°C to -70°C. Reconstituted IL-12 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
