[CD Bioparticles] Clipos™ DOTAP Liposomes

[CD Bioparticles] Clipos™ DOTAP Liposomes

 

 

 

Catalog: CDECDEP-1647

Unit Size: 5 mL

 

 

Specifications

Description	Plain liposomes are extensively used as artificial cell models and for studying the interaction of various types of molecules such as peptides and proteins with the surface of the lipid membranes. Plain liposomes are also used as control formulations for many different types of drug encapsulated liposome formulations. Charged liposomes are also used in many different types of blood complement studies. Plain liposomes are available in various anionic, cationic and neutral surface charges. Plain liposomes can be unilamellar or mulitamellar. DOTAP-based Liposomes use DOPC as a matrix lipid and…More >
Lipid composition	DOTAP/DOPC (40/60 molar ratio)
Liposome Size	100 nm
Lipid Molar Concentration	10 mM
Appearance	White translucent liquid made of nano size unilamellar liposomes. Usually due to the small size of liposomes no settling will occur in the bottom of the vial. The liposomes are packaged in an amber vial.
Buffer	Phosphate Buffered Saline, pH 7.4
Storage	Should always be stored at in the dark at 4 °C, except when brought to room temperature for brief periods prior to animal dosing. DO NOT FREEZE. If the suspension is frozen, the encapsulated drug can be released from the liposomes thus limiting its effectiveness. In addition, that the size of the liposomes will also change upon freezing and thawing.
Shelf-life	4 months.
Technical Notes	Liposomes are unilamellar and sized to 100 nm. If you need them to be made in another size then you should mention that at the time of ordering. Unilamellar liposomes can be made in 50 nm and 200 nm sizes. Liposomes that are larger than 200 nm are multilamellar structure. Liposomes are made in PBS buffer at pH 7.4 but it can be made in any other buffer of your choice. You can specify your buffer at the time of ordering. Liposomes are made in degassed buffer that is purged with argon to avoid oxidation of the unsaturated phospholipids. Liposomes are made under sterile condition. Liposomes are unilamellar and therefore due to their small size they will not settle in the bottom of the vial. If you need to take multiple aliquots out of the vial during a period of time, then it is advised to take extreme care in not contaminating the vial. It is recommended to handle the vial under a sterile hood to maintain the sterility of the product. Liposomes should never be frozen. Crystal ice that is formed during freezing will rupture the lipid membrane of the liposomes and change the size the liposomes particles.