[Transgen Biotech] FlyCut® EcoRI

[Transgen Biotech] FlyCut® EcoRI

 

 

 

Catalog Number：JE201-01

 

FlyCut® EcoRI is expressed and purified from E.coli that carries the recombinant EcoRI gene. The molecular weight is 32.0 kDa, with the recognition site at G^AATTC. The reaction is conducted for 5-10 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm methylation, but sensitive to mammalian CpG methylation.

 

 

Recognition Site
5’…G↓AATTC…3’
3’…CTTAA↑G…5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

Product Contents

Component	JE201-01	JE201-02
FlyCut® EcoRI	5,000 units	2×5,000 units
10×FlyCut® Buffer	2×1 ml	3×1 ml
10×DNA Loading Buffer	1 ml	1 ml