[ProCellSystem] Mouse Hepatic Parenchymal Cell Isolation and Culture Kit

[ProCellSystem] Mouse Hepatic Parenchymal Cell Isolation and Culture Kit

 

 

Cat. No.: P-CA-707

 

 

Volume	3Tests/10Tests
Valid Period	12 months
Shipping Condition	Ice bag

 

Kit Components

Name	Size	Appearance	Storage and Expiration Dat
Pre-perfusion Solution For Mouse Hepatic Parenchymal Cells	3Tests/10Tests	125 mL/400 mL	2-8℃, 1 year
Diluent Of Perfusion Digestive Enzymes For Mouse Hepatic Parenchymal Cells	3Tests/10Tests	125 mL/400 mL	2-8℃, 1 year
Perfusion Digestive Enzyme For Mouse Hepatic Parenchymal Cells	3Tests/10Tests	600 μL/2 mL	-5~-20℃, 1 year
Specialized Washing Solution For Mouse Hepatic Parenchymal Cells	3Tests/10Tests	250 mL/500 mL	2-8℃, 1 year
Specialized Isolation Solution For Mouse Hepatic Parenchymal Cells	3Tests/10Tests	75 mL/250 mL	2-8℃, 1 year
Coating Buffer For Hepatic Parenchymal Cells	3Tests/10Tests	20 mL/50 mL	2-8℃, 1 year
Basic Culture Medium A For Mouse Hepatic Parenchymal Cells	3Tests/10Tests	75 mL/250 mL	2-8℃, 1 year
Supplement A For Mouse Hepatic Parenchymal Cells	3Tests/10Tests	7.5 mL/25 mL	-5~-20℃, 1 year
Basic Culture Medium B For Mouse Hepatic Parenchymal Cells	3Tests/10Tests	150 mL/500 mL	2-8℃, 1 year
Supplement B For Mouse Hepatic Parenchymal Cells	3Tests/10Tests	15 mL/50 mL	-5~-20℃, 1 year
70 μm Cell Filter	3Tests/10Tests	3 pcs/10 pcs	Room temperature, 3 years

Documents

 

Matters Need Attention

1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. In this experiment, you need to prepare PBS buffer, trypan blue staining solution, syringes, scissors, forceps, culture vessels, venous indwelling needle, and other reagents and consumables. If the cultivation needs to be expanded, please prepare complete medium independently.
3. If the isolation process of primary hepatocytes is not handled properly, contamination can occur easily. Ensure that all equipment used in the experiment is sterile.
4. Primary hepatocytes cannot be passaged. Do not use various digestive enzymes to digest and passage the fully confluent hepatocytes after extraction.
5. Hepatocytes are sensitive to fluid shear forces. During the isolation process, avoid vortexing or excessive pipetting when resuspend the cell pallet, in order not to affect cell viability.
6. Liver tissue perfusion can be performed by peristaltic pumps, infusion machines and manual operation. During the process, pay attention to controlling the perfusion flow rate to avoid incomplete or excessive tissue digestion. The following steps take peristaltic pumps and manual perfusion as examples, and the perfusion method can be adjusted according to specific laboratory conditions.
7. The operation of mouse liver perfusion digestion is complicated. Prior to formal experiments, it is recommended to practice pre-perfusion to familiarize yourself with operational procedures and avoid unnecessary waste.
8. Please note that during storage, the Specialized Isolation Solution For Mouse Hepatic Parenchymal Cells in this kit may exhibit crystallization of particulate matter at the bottle mouth or cloudiness in the solution. These are normal phenomenon and the product can be safely used.
9. Due to the bloody process of mouse perfusion, this manual does not provide actual photos for reference, but only schematic diagram. If necessary, please contact the technical support to obtain actual photos of the experimental process.

 

This kit is based on an improved classical perfusion digestion method for isolating and extracting mouse hepatic parenchymal cells. After each experiment (per T), at least 1 ×10⁷ mouse hepatic parenchymal cells can be obtained, following the perfusion digestion of liver tissue from one mouse, as well as the isolation and purification. Immunofluorescence identification shows that the cell purity (CK-18 positive rate) is > 90%.