[IonOpticks] Aurora Ultimate 25×75 C18 UHPLC column

[IonOpticks] Aurora Ultimate 25×75 C18 UHPLC column

 

AUR3-25075C18 (Single)

 

 

Aurora Ultimate 25×75 C18 UHPLC column

25 cm x 75 μm C18 UHPLC packed emitter column with nanoZero® technology, compatible with Nanospray Flex source.

 

Analytical column

Reversed-phase

UHPLC

25 cm

75 µm

120 Å

>1700 bar

60°C

1.7 µm

1–8

C18

 

The original game-changer.

The pinnacle of chromatography performance while minimizing overall cost and stress on equipment. Want it all? The Aurora Ultimate™ continues the legacy of IonOpticks’ original 25 cm UHPLC line, delivering maximum peak capacity when time is less of a determining factor, but still a consideration.

Product Benefits:

• Extreme coverage and sensitivity without excessive time
• Unlocks the true power of your mass spectrometer

Spectacular protein IDs.

Every run. Every time.

Deep proteome coverage.

Maximise the number of identifications that you can achieve from a single shot sample analysis.

Figures 1, 2 & 3: Identified unique proteins, peptides and average Full Width at Half Maximum (FWHM) from 200 ng HeLa Tryptic Digest injection on an Aurora Ultimate™ 25 cm x 75 μm column using different gradient lengths. Samples were run on a Waters ACQUITY UPLC M-Class coupled to a Bruker timsTOF Pro, dia-PASEF acquisition. Data analysed using DIA-NN.

Figure 4: Yeast tryptic digest was spiked into a HeLa tryptic digest (200 ng) in different ratios (Sample A – 45 ng; Sample B – 15 ng). Samples were run on a Waters ACQUITY UPLC M-Class coupled to a Bruker timsTOF Pro, dia-PASEF acquisition using an Aurora 25 cm x 75 μm column. Data analysed using DIA-NN. Each point represents a unique protein with ratios calculated between the A and B samples. Figure 5: Boxplot demonstrating average ratio and interquartile range of data. Expected ratios are indicated with dashed lines.

High quantitative accuracy

Identify large numbers of unique proteins without compromising quantitative accuracy.