TrialtusBioscience18 [Trialtus Bioscience] Gentle Elution Buffer [Trialtus Bioscience] Gentle Elution Buffer Gentle Elution Buffer allows for elution of CL7-tagged proteins from Im7 resin and/or regeneration of Im7 resin without denaturing effects. The high salt and near-neutral pH of Gentle Elution Buffer maintains the native structure of the target protein and the Im7 resin ligand without need for subsequent refolding steps. The eluted protein remains fused.. 2023. 6. 21. [Trialtus Bioscience] Purified Human Growth Hormone (hGH) [Trialtus Bioscience] Purified Human Growth Hormone (hGH) 40-2030 (1mg) SPECIFICATIONS PURITY | >99% (SDS-PAGE gel analysis) ENZYME SOURCE | TriAltus hGH is human recombinant protein produced from E. coli in soluble form purified using our CLiM technology. EC50 (half-maximal effective concentration) | ~0.2 ng/mL measured by a cell proliferation assay using 32D-rGHR cells . ENDOTOXIN | 2023. 6. 8. [Trialtus Bioscience] PreScission Protease [Trialtus Bioscience] PreScission Protease 1mg / 30-2030 The PreScission protease (PSC) recognizes and cleaves the sequence LEVLFQ/GP (where “/” indicates the cleavage site) to release the target protein from the Im7-bound CL7 tag. PURITY | >99% (SDS gel analysis) ENZYME SOURCE | Human Rhinovirus (HRV) 3C Protease overexpressed in E. coli with a glutathione S-transferase (GST) tag CLEAVAGE SITE .. 2023. 5. 15. [Trialtus Bioscience] Im7 4B Gravity Flow Column [Trialtus Bioscience] Im7 4B Gravity Flow Column 11-2060(1mL) 11-2065(5mL) Gravity flow columns are packed with our Im7 4B Resin for use with proteins tagged with CL7. Columns are available in 1 ml and 5 ml sizes. Resin upholds its effectiveness for multiple reactivations. TriAltus’ Im7 4B Resin retains a 1:1 molar binding ratio during repeated uses. The Im7 4B Resin has been used to purify a va.. 2023. 5. 4. [Trialtus Bioscience] SUMO Protease [Trialtus Bioscience] SUMO Protease SKU | 1 mg: 30-1030 5 mg: 30-1035 10 mg: 30-1040 50 mg: 30-1046 100 mg: 30-1050 The SUMO protease releases target proteins from TriAltus’s Im7 resin. This protease recognizes SUMO tertiary structure – not a specific amino acid sequence – to cleave and release the target protein from the Im7-bound CL7 tag. PURITY | ~100% (SDS gel analysis) ENZYME SOURCE | Yeast.. 2023. 4. 20. [Trialtus Bioscience] Im7 4B Resin [Trialtus Bioscience] Im7 4B Resin SKU | 1 mL: 11-1060 2 mL: 11-1062 5 mL: 11-1065 15mL: 11-1071 The Im7 Resin contains a highly specific, resilient Im7 domain that binds the CL7 tag (~16 kDa) fused to target proteins. Following cleavage with the appropriate protease, the target protein releases from the Im7-bound CL7. Available in 1-, 2-, 5-, and 15-mL volumes of settled resin (50% suspension)... 2023. 4. 10. [Trialtus Bioscience] Plasmid #34 [Trialtus Bioscience] Plasmid #34 SKU: 20-1034 EXPRESSION Transcription is induced with IPTG and driven by the T7 RNA polymerase. The plasmid is designed for expression in E. coli. PEPTIDE TAG The C-terminal CL7 tag is downstream of Sortase A (SRT) and PreScission protease (PSC) cleavage sites and a His8 tag. CLEAVAGE SITE(S) N-terminal SUMO and C-terminal SRT and PSC cleavage sites are availabl.. 2023. 4. 4. [Trialtus Bioscience] Starter Kit: 1-mL Im7 4B Gravity Flow Column [Trialtus Bioscience] Starter Kit: 1-mL Im7 4B Gravity Flow Column The 1-mL Im7 4B Resin Gravity Flow Column Starter Kit provides all essential buffers and reagents necessary for the purification of CL7 tagged proteins. Please select a plasmid and an elution protease. -- The Im7 4B 1-mL Gravity Flow Column Starter Kit consists of: Equilibration, Lysis, Elution Buffer (100 mL) Wash Buffer A1 (Hig.. 2023. 3. 27. 이전 1 2 3 다음
