TrialtusBioscience18 [Trialtus Bioscience] Starter Kit: 1-mL Im7 4B Gravity Flow Column [Trialtus Bioscience] Starter Kit: 1-mL Im7 4B Gravity Flow ColumnThe 1-mL Im7 4B Resin Gravity Flow Column Starter Kit provides all essential buffers and reagents necessary for the purification of CL7 tagged proteins. Please select a plasmid and an elution protease.--The Im7 4B 1-mL Gravity Flow Column Starter Kit consists of:Equilibration, Lysis, Elution Buffer (100 mL) Wash Buffer A1 (High S.. 2024. 5. 3. [Trialtus Bioscience] Plasmid #01 [Trialtus Bioscience] Plasmid #01 SKU: 20-1001 EXPRESSION Transcription is induced with IPTG and driven by the T7 RNA polymerase. The plasmid is designed for expression in E. coli. PEPTIDE TAG The CL7 tag for purification is fused to the C-terminus of the third subunit of the ttRNAP protein. CLEAVAGE SITE(S) Plasmid #1 includes several PreScission protease (PSC) cleavage sites. One is at the N-t.. 2024. 2. 2. [Trialtus Bioscience] Im7 4B FPLC Column [Trialtus Bioscience] Im7 4B FPLC Column SKU | 1 mL: 11-3060 5 mL: 11-10365 FPLC columns are packed with our Im7 4B Resin for use with proteins tagged with CL7. Columns are available in 1 ml and 5 ml sizes. Resin upholds its effectiveness for multiple reactivations. TriAltus’ Im7 4B Resin retains a 1:1 molar binding ratio during repeated uses. The Im7 4B Resin has been used to purify a variety o.. 2023. 11. 3. [Trialtus Bioscience] Im7 6B Resin [Trialtus Bioscience] Im7 6B Resin SKU | 1 mL: 10-1060 2 mL: 10-1062 5 mL: 10-1065 15 mL: 10-1071 BUFFER | 20 mM Tris-Cl pH 8.0, 0.5M NaCl, 5% glycerol, 0.05% sodium azide PARTICLE SIZE | Crosslinked agarose 6B beads (45 – 165 μM) PH STABILITY | The Im7 protein is stable on the beads at pH 3-10. However, CL7/Im7 binding is stable at pH 4.2 – 10 only. SALT STABILITY | ≤ 4M NaCl tested BINDING CAP.. 2023. 10. 13. [TrialTus Bioscience] SUMO Protease [TrialTus Bioscience] SUMO Protease SKU | 1 mg: 30-1030 5 mg: 30-1035 10 mg: 30-1040 50 mg: 30-1046 100 mg: 30-1050 The SUMO protease releases target proteins from TriAltus’s Im7 resin. This protease recognizes SUMO tertiary structure – not a specific amino acid sequence – to cleave and release the target protein from the Im7-bound CL7 tag. PURITY | ~100% (SDS gel analysis) ENZYME SOURCE | Yeast.. 2023. 10. 5. [Trialtus Bioscience] BacMam CT eGFP-CL7-H8 [Trialtus Bioscience] BacMam CT eGFP-CL7-H8 SKU: 20-4100 BacMam CT eGFP-CL7-H8 enables target proteins to be labeled with C-terminal eGFP, CL7, and His8 tags. Protease cleavage removes all tags. EXPRESSION The plasmid is designed for transient expression in mammalian cells or for baculovirus transduction of mammalian cells (BacMam). Expression is constitutive and driven by the CMV promoter. A Ko.. 2023. 9. 21. [Trialtus Bioscience] Purified Human Growth Hormone (hGH) [Trialtus Bioscience] Purified Human Growth Hormone (hGH) 40-2030 SKU | 1 mg: 40-2030 5 mg: 40-2035 TriAltus’ recombinant human growth hormone (hGH) is used for a broad range of non-therapeutic research purposes. hGH is produced from E. coli in soluble form and purified using our CLiM technology. hGH is mostly insoluble, but the CL7 tag increases its solubility and avoids the need to purify from.. 2023. 9. 7. [Trialtus Bioscience] Purified Human Growth Hormone (hGH) [Trialtus Bioscience] Purified Human Growth Hormone (hGH) SKU | 1 mg: 40-2030 5 mg: 40-2035 SPECIFICATIONS PURITY | >99% (SDS-PAGE gel analysis) ENZYME SOURCE | TriAltus hGH is human recombinant protein produced from E. coli in soluble form purified using our CLiM technology. EC50 (half-maximal effective concentration) | ~0.2 ng/mL measured by a cell proliferation assay using 32D-rGHR cells . EN.. 2023. 7. 28. 이전 1 2 3 다음
