[FenicsBio] Lentiviral RFP-miR-shRNA cloning vector (tet, Puro)

[FenicsBio]  Lentiviral RFP-miR-shRNA cloning vector (tet, Puro)

 

Catalog number

AB1063

 

Vector name	pLV-tet-RFP-miR-PGK-Puro
Package Size	10 ug
E.coli selection	Amp
Promoter for target gene	TRE
Mammalian selection	Puromycin
Promoter for selection gene	PGK
Storage	-20°C

 

Features: 

• A third generation lentiviral vector
• Extremely low recombination rate
• Compatible with both 2nd and 3rd generation packaging systems, ideal with our Lentivirus packaging system

 

Lentiviral RFP-miR-shRNA cloning vector (tet, Puro) is a third generation lentiviral vector, with tetracycline-responsive promoter element (TRE) to drive TurboRFP and miR-shRNA coexpression and PGK promoter to drive selection marker puromycin expression. This vector is compatible with both 2nd and 3rd generation lentivirus packaging systems.

Clone your miR-shRNA into the Lentiviral RFP-miR-shRNA cloning vector (tet, Puro), then co-transfect with selected Lentivirus Packaging System into packaging cells, such as HEK293T cells, to produce lentivirus. Lentivirus transduced cells can be selected by puromycin to generate stable cell lines.