[Zeta Corporation] IgA (Poly) Rabbit Polyclonal Antibody

[Zeta Corporation] IgA (Poly) Rabbit Polyclonal Antibody

 

 

 

Z2211RL 1.0 ml (Concentrate)
Z2211RS 0.5 ml (Concentrate)
Z2211RT 0.1 ml (Concentrate)
Z2211RP 7 mL (pre‐dilute)

 

Intended Use

This antibody is intended for in vitro diagnostic (IVD) use. IgA (Poly) Rabbit Polyclonal Primary Antibody is intended for laboratory professional use in the detection of the IgA protein in formalin‐fixed, paraffin‐embedded tissue stained in manual qualitative immunohistochemistry (IHC) testing. This antibody is intended to be used after the primary diagnosis of tumor has been made by conventional histopathology using non‐immunological histochemical stains.  The results using this product must be interpreted by a qualified pathologist as an aid to diagnosis in conjunction with the patient’s relevant clinical history, other diagnostic tests and proper controls.

 

Summary and Explanation 

Anti‐IgA antibody reacts with surface immunoglobulin IgA alpha chains. It is useful when identifying leukemias, plasmacytomas, and B‐cell lineage derived Hodgkin’s lymphomas. Due to the restricted expression of heavy and light chains in these diseases, demonstration of B‐cell lymphoma/plasmacytoma is aided with this antibody.

 

Principal of Method

IgA (Poly) Rabbit Polyclonal antibody is use with formalin‐ fixed and paraffin‐embedded sections. Pretreatment of deparaffinized tissue with heat‐induced epitope retrieval or enzymatic retrieval is recommended. In general, immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody (link antibody), an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site.  Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

 

Materials Provided

IgA (Poly) Rabbit Polyclonal in concentrated form or prediluted Antibody Specifications: Antibody as Purified antibody diluted in Tris‐HCI buffer containing stabilizing protein and <0.1% sodium azide.   Host: Rabbit Isotype: IgG Immunogen: Purified IgA Cellular Localization: Cytoplasmic. Concentrate Dilution Range: 1:50‐200 Positive control: Lymph node.

 

Storage and Handling

Upon receiving, store vial at 2‐8oC. When stored at 2‐8oC, this antibody is stable for 24 months. To ensure proper reagent stability and functionality, the cap must be replaced and the bottle must be placed in a refrigerator immediately in an upright position. Do not use after the expiration date stamped on the vial. If reagents are stored under any conditions other than those specified in the package insert, they must be verified by the user. Repeat freeze and thaw cycles may decrease antibody activity and should be avoided. If packaging or contents appear to be broken or damaged do not use and contact Zeta Corporation.  Contact information is on the last page of this document.

 

Reconstitution:

Predilute Antibodies: Ready to use, no reconstitution necessary. Concentrate Antibodies: Refer to established dilution range 1:50‐200 and use appropriate lab‐standardized diluent and container. Refer to the label for any specific instructions. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6 months at ‐20°C as demonstrated by stability studies performed at Zeta Corporation when using polypropylene containers.