[AddexBio] 360 MMP FRET Substrate III

[AddexBio] 360 MMP FRET Substrate III

 

ABBFO2064

 

Optimized for both 72-kDa fibroblast gelatinase(MMP 2) and 92-kDa neutrophil gelatinase(MMP 9), this peptide substrate has the fluorescent Trp residue in subsite P'2 and the dinitrophenol (Dnp) quenching group on the N-terminus. The quenching of the Trp fluorescence in the intact substrate is relieved upon hydrolysis of the P1-P'1 bond, giving rise to a continuously recording fluorescence assay. The residues placed in subsites P3-P'1 and P'3 are optimized for MMP, while Arg is placed in P'4 to enhance solubility. The water solubility of this peptide substrate in assay buffer exceeds the Km value for the enzyme reaction, allowing accurate determination of the kinetic parameters. Abs/Em = 280/360 nm.

Technical Data:

M.Wt: 1012.1

Sequence (one-letter code): Dnp-PLGMWSR

Sequence (three-letter code): Dnp - Pro - Leu - Gly - Met - Trp - Ser - Arg - OH

Excitation (nm): 280

Emission (nm): 360

Solubility: Soluble in DMSO

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store and desiccate at -20°C and protect from light.