[Biomax] Quanti-MAX™ WST-8 Cell Viability Assay Kit
| QM1000 - 1000tests QM2500 - 2500tests QM5000 - 5000tests |
Description
Quanti-Max™ WST-8 Cell Viability Assay Kit measures the amount of living cells using WST-8 and can be used for Cell Viability, Proliferation & Cytotoxicity Assay. The key component, WST-8, is a highly water-soluble Tetrazolium Salt that reacts with dehydrogenase in living cells to form an orange-colored, water-soluble formazan product. Dehydrogenase is an enzyme that exists in the mitochondrial electron transport chain of metabolically active cells and is active only in living cells.
Thus, the production of formazan is linearly correlated with the number of living cells, which can be determined by measuring the absorbance (450nm). Quanti-Max™ WST-8 cell viability assay kit is a sterilized single bottle solution that can be used without preparation beforehand, eliminating the process of melting formazan and the need to remove culture media, allowing the user to easily test the suspension cell.
It is refrigerated in 0~4℃ and can be used without change of activity for 1 year from the date of manufacture. It can be used for more than 2 years when stored at -20℃. However, when the product is frozen and thawed repeatedly, the activity may reduce and the background may increase. Because this product is sensitive to light, store in a container that blocks light or in a container covered with aluminum foil.
Check out the video to learn more about the Quanti-MAX WST-8 Cell Viability Assay Kit
Assay procedure compared with MTT
Sensitivity & Stability
High SensitivityComparison of sensitivity for each other test reagent.
[ Colon26-M3.1 (Carcinoma cells), Gibco DMEM with 10% FBS ]
Long stabilityQuanti-MAX™ is stable at 4℃ for 1 yr.
Protocols
100 μl of the culture medium is to be used for the experiment and 10 μl of Quanti-Max™ is mixed and used as a blank. (Cell-free culture medium + Quanti-Max™). Even when Quanti-Max™ is added to a cell-free culture medium, a weak absorbance can be measured due to the type of medium, incubation time, and exposure to light.
1. Prepare the cell culture medium and dispense 100 μl per well into a 96-well plate and incubate for 24 hours in a CO2 incubator.
2. Add 10 μl of test material (e.g., toxicant) to each well at various concentrations.
3. React in an incubator for the appropriate time to meet the experimental conditions.
4. Add 10 μl of Quanti-Max™ to each well.
5. React in an incubator for 0.5 to 4 hours
6. Shake gently for 1 minute before measuring the absorbance.
7. Measure the absorbance at 450 nm using a plate reader.
